Investigation of the in vivo estrogenicity of the UV-filters 4-methylbenzylidene camphor and octyl methoxy cinnamate in rainbow trout (Oncorhynchus mykiss)

The estrogenic activity of the chemical UV-filters, 4-methylbenzylidene camphor (4-MBC) and octyl methoxy cinnamate (OMC) was investigated in an vivo rainbow trout ( Oncorhynchus mykiss ) assay. Plasma vitellogenin concentrations were quantified by means Enzyme Linked Immuno Sorbent Assay (ELISA) juvenile before after intraperitoneal injection test compounds. Injection 4-MBC on day 0, 3, 6 10 exposure period caused dose time dependent increases concentration plasma vitellogenin. Significant elevation demonstrated from 151 mg kg −1 . Logistic regression analysis relating percentage responding fish to injected resulted ED , 50 90 values 37, 115 194 respectively, 14 days (4 injections). Injections with OMC (up 202 did not result a statistically significant response groups exposed fish, although some individual showed elevated plasma. results confirm that is vivo. • effect tested Vitellogenin induction used as biomarker injection. induced synthesis 4-MBC/kg/injection. OMC/kg increased average concentrations. However, few responded OMC/kg.